Results| Tuesday, 4 August 2009 |
| q1l1 |  Universidad de Chile | 2-1 | Cali |
|
| Wednesday, 5 August 2009 |
| q1l1 | La Equidad | 2-2 | Unión Española |
|
| q1l1 |  Zamora | 0-1 | Emelec |
|
| Thursday, 6 August 2009 |
| q1l1 |  Alianza Atlético | 0-0 | Anzoátegui* |
|
| Tuesday, 11 August 2009 |
| q1l1 |  Blooming | 0-3 | River Plate |
|
| Score awarded as 0-3. |
| q1l1 | LDU Quito | 1-0 | Libertad |
|
| Wednesday, 12 August 2009 |
| q1l1 | Cerro Porteño | 2-0 | La Paz FC |
|
| q1l1 |  Fluminense | 0-0 | Flamengo |
|
| Thursday, 13 August 2009 |
| q1l1 | Liverpool | 0-0 | Cienciano |
|
| q1l1 | Vitória | 2-0 | Coritiba |
|
| Tuesday, 18 August 2009 |
| q1l1 |  Tigre | 2-1 | San Lorenzo |
|
| q1l2 | Cali | 0-1 | Universidad de Chile | 1-3 |
| Wednesday, 19 August 2009 |
| q1l1 | River Plate | 1-2 | Lanús |
|
| Thursday, 20 August 2009 |
| q1l1 | Boca Juniors | 1-1 | Vélez Sársfield |
|
| q1l2 | La Paz FC | 1-2 | Cerro Porteño | 1-4 |
| q1l2 | Cienciano | 2-0 | Liverpool | 2-0 |
| Tuesday, 25 August 2009 |
| q1l2 | Libertad | 1-1 | LDU Quito | 1-2 |
| q1l2 | Unión Española | 1-0 | La Equidad | 3-2 |
| q1l2 | Coritiba | 2-0 | Vitória | 2-2, aet, p3-5 |
| Wednesday, 26 August 2009 |
| q1l1 | Atlético-MG | 1-1 | Goiás |
|
| q1l2 | Flamengo | 1-1 | Fluminense | 1-1 |
| Thursday, 27 August 2009 |
| q1l2 | River Plate | 2-1 | Blooming | 3-1 |
| q1l2 | Emelec | 2-1 | Zamora | 3-1 |
| Wednesday, 2 September 2009 |
| q1l1 | Athlético-PR | 0-0 | Botafogo |
|
| Tuesday, 15 September 2009 |
| q1l2 | San Lorenzo | 1-0 | Tigre | 2-2 |
| q1l2 | Anzoátegui* | 1-2 | Alianza Atlético | 1-2 |
| Wednesday, 16 September 2009 |
| q1l2 | Vélez Sársfield | 1-0 | Boca Juniors | 2-1 |
| q1l2 | Botafogo | 3-2 | Athlético-PR | 3-2 |
| q1l2 | Goiás | 1-1 | Atlético-MG | 2-2, aet, p6-5 |
| Thursday, 17 September 2009 |
| q1l2 | Lanús | 1-0 | River Plate | 3-1 |
