MLS NEXT Pro| ROUND | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| Season archive |
| Thursday, 18 September 2025 | |||||
| New York City II | - | New England Revolution 2 | |||
| Friday, 19 September 2025 | |||||
| Toronto FC 2 | - | Columbus Crew 2 | |||
| Sporting Kansas City 2 | - | Vancouver Whitecaps 2 | |||
| Austin FC II | - | St. Louis CITY SC 2 | |||
| Los Angeles II | - | Real Monarchs | |||
| Saturday, 20 September 2025 | |||||
| Crown Legacy | - | Atlanta United 2 | |||
| Sunday, 21 September 2025 | |||||
| Ventura County | - | The Town | |||
| Tacoma Defiance | - | Colorado Rapids 2 | |||
| New York Red Bulls 2 | - | Inter Miami II | |||
| Chattanooga FC | - | Carolina Core | |||
| Orlando City B | - | Huntsville City | |||
| Philadelphia Union 2 | - | Chicago Fire II | |||
| FC Cincinnati 2 | - | New England Revolution 2 | |||
| Houston Dynamo 2 | - | Portland Timbers 2 | |||
| Minnesota United 2 | - | North Texas | |||
