| RESULTS | | Wednesday, 18 February 2009 | | r1l1 | Americano | 2-0 | Santa Cruz |
| | r1l1 | Americana | 2-0 | Caxias |
| | r1l1 | Misto | 1-1 | Campinense |
| | r1l1 | J. Malucelli | 0-2 | Guarani |
| | r1l1 | Flamengo-PI | 1-4 | Vasco da Gama |
| | r1l1 | Nacional-PB | 0-1 | Fluminense |
| | r1l1 | Rio Branco | 1-2 | Santos |
| | r1l1 | Desportiva | 1-1 | Fortaleza |
| | r1l1 | Holanda | 1-2 | Coritiba |
| | r1l1 | Itabaiana | 0-5 | Atlético-MG |
| | r1l1 | Tupi | 2-0 | Criciúma |
| | r1l1 | Mixto | 1-2 | Paraná |
| | r1l1 | Icasa | 1-1 | Portuguesa |
| | r1l1 | União-MT | 1-0 | Internacional |
| | r1l1 | Sampaio Corrêa | 3-2 | Figueirense |
| | Wednesday, 4 March 2009 | | r1l1 | Confiança | 3-2 | América-RN |
| | r1l1 | Fast | 1-1 | ABC |
| | r1l1 | Águia Marabá | 2-1 | América-MG |
| | r1l1 | Serra | 2-3 | CSA |
| | r1l1 | Potiguar | 2-2 | Bahia |
| | r1l1 | Barras | 0-1 | Remo |
| | r1l1 | ASA | 1-1 | Vitória |
| | r1l1 | Tocantins | 0-3 | Atlético-PR |
| | r1l1 | Atlético Sorocaba | 1-0 | Juventude |
| | r1l1 | Central-PE | 0-0 | Ceará |
| | r1l1 | Itumbiara-GO | 0-2 | Corinthians |
| | r1l1 | Atlético Roraima | 1-3 | Goiás |
| | r1l1 | Cristal-AP | 1-2 | Brasiliense |
| | r1l1 | Vilhena | 1-2 | Ponte Preta |
| | r1l1 | Moto Clube | 1-1 | Náutico |
| | r1l1 | Ivinhema | 0-5 | Flamengo |
| | r1l2 | Caxias | 2-1 | Americana | 2-3 | | r1l2 | Criciúma | 3-0 | Tupi | 3-2 | | r1l2 | Coritiba | 3-0 | Holanda | 5-1 | | r1l2 | Fortaleza | 3-0 | Desportiva | 4-1 | | r1l2 | Atlético-MG | - | Itabaiana | 5-0 | | r1l2 | Guarani | - | J. Malucelli | 2-0 | | r1l2 | Vasco da Gama | - | Flamengo-PI | 4-1 | | r1l2 | Internacional | 2-0 | União-MT | 2-1 | | r1l2 | Figueirense | 2-1 | Sampaio Corrêa | 4-4 | | r1l2 | Campinense | 1-1 | Misto | 2-2, p3-4 | | r1l2 | Portuguesa | 1-1 | Icasa | 2-2, p3-4 | | Thursday, 5 March 2009 | | r1l1 | Dom Pedro II | 0-2 | Botafogo |
| | r1l2 | Santa Cruz | 2-4 | Americano | 2-6 | | r1l2 | Fluminense | 3-0 | Nacional-PB | 4-0 | | r1l2 | Paraná | 1-2 | Mixto | 3-3, p4-2 | | Wednesday, 18 March 2009 | | r1l2 | CSA | 3-1 | Serra | 6-3 | | r1l2 | Remo | 2-0 | Barras | 3-0 | | r1l2 | Brasiliense | 3-1 | Cristal-AP | 5-2 | | r1l2 | Ceará | 1-1 | Central-PE | 1-1 | | r1l2 | Juventude | 2-0 | Atlético Sorocaba | 2-1 | | r1l2 | Náutico | 2-0 | Moto Clube | 3-1 | | r1l2 | Santos | 4-0 | Rio Branco | 6-1 | | r1l2 | Ponte Preta | 6-1 | Vilhena | 8-2 | | r1l2 | América-RN | 0-0 | Confiança | 2-3 | | r1l2 | Atlético-PR | - | Tocantins | 3-0 | | r1l2 | Flamengo | - | Ivinhema | 5-0 | | r1l2 | Goiás | - | Atlético Roraima | 3-1 | | r1l2 | Vitória | 1-1 | ASA | 2-2, aet, p5-4 | | Thursday, 19 March 2009 | | r1l2 | América-MG | 0-1 | Águia Marabá | 1-3 | | r1l2 | Bahia | 6-1 | Potiguar | 8-3 | | r1l2 | ABC | 4-0 | Fast | 5-1 | | r1l2 | Botafogo | - | Dom Pedro II | 2-0 | | r1l2 | Corinthians | - | Itumbiara-GO | 2-0 |
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Copa do Brasil